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IQFISH fast hybridization buffer is a formamide-free hybridization buffer that enables hybridization to be completed in 1-1.5 hours. IQFISH buffer is provided in three different size formats to meet the needs of varying testing volumes.
IQFISH Fast Hybridization Buffer is a formamide-free hybridization buffer that enables ISH hybridization to be completed in 1-1.5 hours. A complete in situ hybridization (ISH) assay on a formalin-fixed, paraffin-embedded (FFPE) sample can be completed in 4 hours enabling a half day sample-to-result workflow. This workflow offers many operation advantages such as increased turnaround time, same day repeat testing without impacting the quality of hybridization.|Standard formamide-based FISH hybridization buffer lowers the melting temperature of nucleic acids by targeting hydrogen bonds and interfering with DNA base pairing resulting in the denaturation of the DNA double strand. The interference slows down the following base pairing between the FISH probe and specimen DNA. However, IQFISH buffer destabilizes the DNA helix by diminishing hydrophobic base stacking (1). Minimized based paring interference enables fast binding between the ISH probe and specimen DNA.|IQFISH buffer is provided in three different size formats to meet the needs of varying testing volumes.
This is the data sheet for IQFISH Fast Hybridization buffer including part numbers G9414A, G9415A, G9416A
IQFISH Fast Hybridization Buffer Package Insert in English, French, and German
Dako Omnis Vial with Mixing Ball, 2 mL has been designed as an accessory for Dako Omnis and Dako Omnis Mixing Device and is intended for use in ISH procedures using user-provided FISH probes diluted in ethylene carbonate-based hybridization buffer...
Dako Omnis Vial with Mixing Ball, 2 mL has been designed as an accessory for Dako Omnis and Dako Omnis Mixing Device and is intended for use in ISH procedures using user-provided FISH probes diluted in ethylene carbonate-based hybridization buffer (IQFISH). Dako Omnis Vial with Mixing Ball, 2 mL includes a mixing ball that is used by Dako Omnis Mixing Device to mix the IQFISH hybridization buffer with the user-provided probe. Each package contains 25 vials, 25 caps and 25 mixing balls.