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A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.
- Publication Part Number: 5994-6123EN
- Created: 01 Oct 2023
- 36 MB
This application note describes multi-attribute analysis of monoclonal antibodies using the Agilent InfinityLab 2D-LC Solution and the Agilent 6530 LC/Q-TOF system.
- Publication Part Number: 5994-3521EN
- Created: 11 May 2021
- 6 MB
Presents the use of the AdvanceBio HIC column to resolve monoclonal antibody variants from their native form without mAb digestion into subunits.
- Publication Part Number: 5994-0199EN
- Created: 10 July 2019
- 628 KB
Robust Hydrophobic Interaction Chromatography of Intact Native Proteins, Agilent AdvanceBio HIC columns
- Publication Part Number: 5991-9180EN
- Created: 21 Aug 2018
- 2 MB
Describes the use of the new AdvanceBio HIC column, with method optimization, to assess DAR values of ADCs.
- Publication Part Number: 5994-0149EN
- Created: 31 July 2018
- 831 KB
HIC is a key technique for purification of proteins.The AdvanceBio HIC column showed excellent correlation with protein hydrophobicity (GRAVY), and retention time ensuring predictable elution behavior.
- Publication Part Number: 5994-5252EN
- Created: 08 Sep 2022
- 743 KB
Workflow solutions for Optimizing Native ADC and mAb Analysis.
- Publication Part Number: 5994-2098EN
- Created: 01 June 2020
- 9 MB
A Trio of Techniques to Complete CQA Characterization.
- Publication Part Number: 5994-2097EN
- Created: 01 June 2020
- 8 MB
Technical poster on intact analysis of biopharmaceuticals by hydrophobic interaction/reversed-phase 2D-LC/MS
- Publication Part Number: 5994-1162EN
- Created: 07 July 2019
- 524 KB
Poster that was presented at HPLC 2018: Hydrophobic Interaction Chromatography- Native Mode Separation of Bio-Therapeutic Workflows
- Publication Part Number: 5994-0200EN
- Created: 08 Aug 2018
- 548 KB