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Technical overview describing ion exchange method development and troubleshooting
- Publication Part Number: 5994-8708EN
- Created: 11 Nov 2025
- 466 KB
This application note highlights excellent performance of Agilent PL-SAX columns for synthetic oligonucleotide analysis and suggests an optimal kickoff for exploring alternative method conditions.
- Publication Part Number: 5994-8381EN
- Created: 22 May 2025
- 570 KB
A guide to find the right column for your characterization workflow. It includes advice and tips on method development, solvent choice, mobile phase modification, optimization, example separations, as well as ordering information.
- Publication Part Number: 5994-6123EN
- Created: 01 Oct 2023
- 36 MB
This application note demonstrates the utility of Agilent Bio SAX columns for oligonucleotide analysis and provides suggested starting points for further method development.
- Publication Part Number: 5994-5198EN
- Created: 13 Mar 2023
- 1 MB
Information and tips on installing and using Agilent PLRP-S, PL-SAX, and PL-SCX preparative LC columns
- Publication Part Number: 5994-4723EN
- Created: 01 Feb 2023
- 171 KB
This application note describes common method parameters that can be optimized when purifying oligonucleotides ranging from 25 to 100 bases in length.
- Publication Part Number: 5994-4753EN
- Created: 01 Apr 2022
- 416 KB
Critical Quality Attributes Charge Variant compendium
- Publication Part Number: 5994-0034EN
- Created: 23 Apr 2021
- 10 MB
Anion-exchange chromatography for the analysis and purification of oligonucleotides using a PL-SAX column.
- Publication Part Number: 5990-8297EN
- Created: 15 Mar 2021
- 313 KB
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Anion Exchange Chromatography with Agilent Bio HPLC columns and 1260 Infinity Bio-Inert LC System
- Publication Part Number: 5991-6106EN
- Created: 14 Aug 2015
- 2 MB
Describes the application of the PL-SAX column for HPLC separations and analysis of different nucleotide and oligonucleotides.
- Publication Part Number: 5990-8779EN
- Created: 10 Aug 2011
- 802 KB
Describes the application of the PL-SAX column for HPLC separations and analysis of proteins and deprotected oligonucleotides under denaturing conditions.
- Publication Part Number: 5990-8778EN
- Created: 09 Aug 2011
- 776 KB
Explains the different packing methods of the PL-SAX column and demonstrates to effects on common proteins as experimental analytes.
- Publication Part Number: 5990-8777EN
- Created: 09 Aug 2011
- 861 KB
Describes the pore structure of PL-SAX 4000? and how it is ideal for high resolution, and high speed applications including the purification of amyloglucosidase.
- Publication Part Number: 5990-8664EN
- Created: 18 July 2011
- 642 KB
Describes the globular protein analysis and puri?cation, using PL-SAX 1000? material to separate ovalbumin & soyabean trypsin inhibitors.
- Publication Part Number: SI-1656
- Created: 20 Oct 2010
- 52 KB
This poster covers columns for oligonucleotide chromatography, from siRNA/ASO to mRNA.
- Publication Part Number: 5994-7567EN
- Created: 07 June 2024
- 12 MB
Explains IEX chromatography, column selection, mobile phase considerations, general rules of thumb for using IEX, and more.
- Publication Part Number: 5991-3775EN
- Created: 02 May 2014
- 9 MB