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This application note presents the SEC method, coupled with LC/UV and LC/Q-TOF, to study the oligomeric states of liraglutide.
Guidance for choosing the appropriate pore size column for oligonucleotide separation by size exclusion chromatography.
Accurately determine biomolecule aggregation and fragmentation with Agilent size exclusion columns.
Comparison of SEC columns for aggregate analysis of AAVs
The Agilent AdvanceBio SEC column together with the Agilent 1260 Infinity II Bio-inert System is an ideal combination for the aggregate analysis of ADCs
Aggregate analysis of adeno-associated viruses with AdvanceBio SEC 500 ? columns
Aggregate analysis of a ~50 nm virus-like particle using an AdvanceBio SEC 1000? column.
The enzyme l-asparaginase poses a challenge for quantifying aggregation as its structure may produce high molecular weight aggregates.
An SEC method identifying innovator and biosimilar insulin analog was developed using an Agilent AdvanceBio SEC 130?, 7.8 × 300 mm, 2.7 μm column.
Evaluation of SEC Columns for Analysis of ADC Aggregates and Fragments - Choosing the best stationary phase to overcome nonspecific interactions.
This collection of 10 Agilent ‘a(chǎn)ggregate and fragment analysis’ app notes also includes our guide on how to perform SEC for biomolecule analysis.
An overview of the separation and characterization of Emicizumab, using a variety of products from the Agilent biocolumns portfolio.
In this application note, we have developed a mass spectrometry-compatible, native-mode size exclusion (SEC/MS) method for characterization of a challenging FDC with very low pI.
Nanobody analysis with AdvanceBio SEC 120 ? 1.9 μm column for size exclusion chromatography and AdvanceBio HIC column hydrophobic interaction chromatography (HIC)
Comparison of AdvanceBio SEC 200 ? 1.9 μm column for SEC analysis of hGH, hG-CSF and IFN alpha-2b therapeutic proteins with sub-2 μm particle columns from other vendors.
Presents a method for the rapid analysis of a monoclonal antibody using the Agilent AdvanceBio SEC 200 ? 19 μm column.
Comparison of HPLC method and a quick method developed using the Cary 60 UV-Vis
This application note describes a robust LC/MS method for the analysis of native protein analysis.
Improvements in resolution and shorter run times using AdvanceBio SEC 120 ? 1.9 μm, 4.6 x 300 mm column for insulin analysis.
Size Exclusion Chromatography Method Development of NIST mAb Using an Agilent AdvanceBio SEC 200 ?, 1.9 μm Column