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This Application Note describes a simple method for the analysis of disulfide linkages in monoclonal antibodies (mAbs) by reversed phase HPLC.
- Publication Part Number: 5991-1694EN
- Created: 15 Oct 2024
- 961 KB
| Publication Part Number | 5991-1694EN | ||
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Small –scale purification of a monoclonal antibody from a cell lysate and the subsequent analysis on a single instrument, the Agilent 1260 Infinity Bio-inert Quaternary LC.
- Publication Part Number: 5991-1195EN
- Created: 01 May 2016
- 854 KB
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This Application Note demonstrates the sensitive and reproducible analysis of N-linked glycans of human immunoglobulin G with a novel HILIC column and fluorescence detection.
- Publication Part Number: 5991-4801EN
- Created: 01 Nov 2015
- 840 KB
| Publication Part Number | 5991-4801EN | |||
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This Application Note describes the quantification of human IgG with the Agilent Bio-Monolith Protein A HPLC Column with alternating column regeneration.
- Publication Part Number: 5991-4723EN
- Created: 01 June 2014
- 836 KB
| Publication Part Number | 5991-4723EN | ||
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In this application note, pre-packed Bio-monolith Protein A columns, part of the AdvanceBio family, were used to demonstrate the quick capture of monoclonal antibody titer
- Publication Part Number: 5991-2990EN
- Created: 08 Jan 2014
- 441 KB
| Publication Part Number | 5991-2990EN | ||
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Agilent Bio MAb columns, in an Agilent 1260 Infinity Bio-inert Quaternary LC, characterize the charge heterogeneity of IgG1monoclonal antibody...
- Publication Part Number: 5991-0895EN
- Created: 01 Aug 2013
- 240 KB
| Publication Part Number | 5991-0895EN | |||
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This shows the separation of four proteins by anion exchange chromatography using 4 different high salt-containing elution buffers (up to 2 M) for linear and step gradients.
- Publication Part Number: 5990-9614EN
- Created: 01 Aug 2013
- 668 KB
| Publication Part Number | 5990-9614EN | |||||
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Ion exchange is a very useful technique to separate protein mixtures under mild conditions, most commonly by applying a shallow gradient of increasing salt concentration as el
- Publication Part Number: 5990-9629EN
- Created: 01 Aug 2013
- 1 MB
| Publication Part Number | 5990-9629EN | |||
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Ion exchange is a commonly used technique for the separation of complex protein mixtures.
- Publication Part Number: 5990-9931EN
- Created: 01 Aug 2013
- 2 MB
| Publication Part Number | 5990-9931EN | ||||
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Columns containing weak cation-exchange stationary phases offer a degree of versability when used for separation of positively charged proteins.
- Publication Part Number: 5990-9628EN
- Created: 01 Aug 2013
- 1 MB
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Methods are given for the use of Agilent Bio-SEC columns, with LS, UV and RI detection, for quantification or quality control of protein aggregates
- Publication Part Number: 5991-1400EN
- Created: 07 Jan 2013
- 284 KB
| Publication Part Number | 5991-1400EN | ||||
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This Application Note describes a high-resolution, pH-based separation of acidic and basic charge variants for monoclonal antibodies using the Agilent 1260 and BiomAb PEEK
- Publication Part Number: 5991-1407EN
- Created: 01 Dec 2012
- 824 KB
| Publication Part Number | 5991-1407EN | ||
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