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An aggregate analysis workflow for SEC mobile phase optimization and characterization of mAb aggregation profiles
"This Application Note describes the quantification of aggregates in biotherapeutic
Agilent AdvanceBioSEC columns used for fast, high resolution, sensitive, and reproducible separation and quantitation of mAbs and Antibody Drug Conjugate,
A SEC method for determining the purity of PEG Granulocyte Colony Stimulating Factor using an Agilent AdvanceBio SEC column with aqueous mobile phase.
Shorter 150 mm AdvanceBio SEC columns and higher flow rates of 1.5 mL/min increase sample throughput by 400%, from 1.3 days to under 8 hours.
Analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS)
High resolution and sensitive size exclusion chromatography separates and quantitates rituximab aggregates/degradants in a bioinert LC with an AdvanceBio SEC column.
Multiple injections of Tween 20 had no effect on the performance of Agilent Bio SEC columns or integration data when analyzing IgG.
Methods are given for the use of Agilent Bio-SEC columns, with LS, UV and RI detection, for quantification or quality control of protein aggregates
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