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The Agilent xCELLigence real-time cell analysis (RTCA) eSight system gives its users the flexibility to use both label-free impedance and/or imaging in four channels for the real-time monitoring of cell proliferation and cytotoxicity. The eSight has...
Robust microplate-based approach for quantifying induced activation of T cells utilizing automated imaging, luminescence, and ELISA.
Here we describe image-based immune cell killing assays of 3D single tumor spheroids expressing RFP using the xCELLigence RTCA eSight.
Recently updated Cancer Immunotherapy Handbook featuring the latest high impact xCELLigence publications and newly released supporting information.
Describes use of a tethering approach to immobilize liquid tumor cell lines on to the surface of gold biosensors embedded in an Agilent E-Plate.
The Agilent BioTek Cytation C10 confocal imaging reader provides a high-throughput kinetic 3D fluorescence microscopy solution to quantify CAR T-mediated cell killing of target spheroids.
Automated brightfield imaging assay enables label-free quantification of T cell and CAR T cell activation through dynamic cluster formation analysis.
This application note demonstrates a robust method for performing real-time antibody internalization assays using the Agilent xCELLigence RTCA eSight combined with CypHer5E pH-sensitive dyes.
Discover Agilent's automated imaging-based method for robust analysis of CAR T cell cytotoxicity, enhancing cancer treatment efficacy and research efficiency.
This poster reveals to optimize T-cell differentiation in bioreactors using integrated Agilent cell analytics and feedback control to enhance CAR T-cell therapyproduction.
CAR T-cell manufacturing conditions are critical to differentiation, metabolic fitness, and cytotoxic function, enhancing therapy efficacy and production efficiency.
Poster presented at AAI 2025. Immune cell-mediated cytotoxicity of CD19 CAR-T cells against Raji suspension tumor cells was monitored and measured using real-time whole-well live cellimaging on the xCELLigence RTCA eSight system with its 5x...
In this study, we present a robust approach to monitor and analyze the bystander effect of Antibody-Drug Conjugates in real-time using live-cell imaging andimpedance measurements with the Agilent xCELLigence RTCA eSight system.
AACR 2025 poster: Chronic anti-CD3/CD28 stimulation during cell expansion generates exhausted T cells with a distinct metabolic and cytotoxic phenotype
AACR 2025 poster: Chronic anti-CD3/CD28 stimulation during cell expansion generates exhausted T cells with a distinct metabolic and cytotoxic phenotypeCAR T cells were tested against breast cancer cells (T47D) over seven rounds to assess...
There is a need for functional potency assays that can be used to rapidly test the efficacy of different immunotherapy approaches, including but not limited tosmall molecules, biologics, cell therapies, or combinations of these agents
The eSight can be used to study cytotoxicity kinetics of lymphocytes invading a deregulated extracellular matrix present in solid tumors.
we demonstrated consistent production of CAR T-cells using the functionally closed and automated cell manufacturing Lonza Cocoon platform, using a novelcombination of cell analysis parameters obtained from an Agilent xCELLigence RTCA system, a...