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Consumables workflow ordering guide - N-glycan analysis of biotherapeutic glycoproteins using AdvanceBio Gly-X 2-AB InstantPC sample preparation and LC/FLD/MS.
In this application note, we profiled the N-glycans of several monoclonal antibodies with the new Agilent AdvanceBio Amide HILIC column.
This application note describes use of the Agilent AdvanceBio sialic acid profiling and quantitation kit (p/n GS24-SAP) to profile sialic acid species present and perform absolute sialic acid quantitation.
AdvanceBio Desalting - RP cartridges used for effective desalting of LC-separated monoclonal antibodies (mAbs) before characterization by mass spectrometry
A versatile Agilent Bio-Monolith Protein A column analyzed an optimized recombinant mAb manufactured with desired structural characteristics.
An Agilent 2.7 μm HILIC column revealed a glycopeptide not seen by RP. HILIC can be orthogonal and complementary to RP for better retention of hydrophilic peptides.
This Application Note shows the workflow solution for the physicochemical and biological characterization of a biosimilar and innovator rituximab.
The charge heterogeneity provides a great challenge for the complete characterization of the monoclonal antibody (mAb).
The Agilent HILIC-based AdvanceBio Glycan Mapping column is 40% faster, with high resolution, efficiency, and chemical stability for at least 5,000 column volumes.
The Agilent 1.8 μm HILIC column reduced run time by 40% when separating N-linked glycans. The column also resolved complex antennary bovine fetuin glycans.
Fast and efficient LC TOF/MS characterizes intact and digested IgG to identify glycoform masses and profiles. mAb-glyco chip analysis built a glycan accurate mass database.
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This Application Note describes a high-resolution, pH-based separation of acidic and basic charge variants for monoclonal antibodies using the Agilent 1260 and BiomAb PEEK
| Publication Part Number | 5991-1407EN | ||
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Agilent offers tools and support for every step of your glycan sample preparation and analysis workflow.
AdvanceBio glycoenzymes are ideal for use with glycan assays, protein modification assays, and sequencing studies when used together.
A technical flyer which provides an overview of the AdvanceBio Sialic Acid Profiling and Quantitation kit, p/n GS24-SAP.
Achieve more streamlined sample processing with the AdvanceBio Sialic Acid quantitation kit.