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This Technical Overview provides some tips and tricks on how to correctly apply the Agilent Buffer Advisor Software for the generation of pH gradients over a wide range
This Application Note describes a simple method for the analysis of disulfide linkages in monoclonal antibodies (mAbs) by reversed phase HPLC.
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Agilent Bio-Monolith Protein G quantitates supernatant mAbs across a wide loading range at many flow rates, complementing Protein A for fast titer analysis.
This application note describes multi-attribute analysis of monoclonal antibodies using the Agilent InfinityLab 2D-LC Solution and the Agilent 6530 LC/Q-TOF system.
An aggregate analysis workflow for SEC mobile phase optimization and characterization of mAb aggregation profiles
"This Application Note describes the quantification of aggregates in biotherapeutic
AdvanceBio Desalting - RP cartridges used for effective desalting of LC-separated monoclonal antibodies (mAbs) before characterization by mass spectrometry
Agilent AdvanceBioSEC columns used for fast, high resolution, sensitive, and reproducible separation and quantitation of mAbs and Antibody Drug Conjugate,
A SEC method for determining the purity of PEG Granulocyte Colony Stimulating Factor using an Agilent AdvanceBio SEC column with aqueous mobile phase.
Shorter 150 mm AdvanceBio SEC columns and higher flow rates of 1.5 mL/min increase sample throughput by 400%, from 1.3 days to under 8 hours.
Analysis of an mAb (trastuzumab) and an ADC (trastuzumab emtansine) by size exclusion chromatography with online mass spectrometry (SEC-MS)
High resolution and sensitive size exclusion chromatography separates and quantitates rituximab aggregates/degradants in a bioinert LC with an AdvanceBio SEC column.
"Fast and High Resolution Analysis of Intact and Reduced Therapeutic Monoclonal Antibodies (mAbs) using the Agilent Bio-inert LC and AdvanceBio RP-mAb Columns
Biomolecule Characterization workflow. Agilent Bio-Monolith Protein A and G Affinity columns. and 1260 Infinity Bio-Inert LC System.
Anion Exchange Chromatography with Agilent Bio HPLC columns and 1260 Infinity Bio-Inert LC System
Reversed-phase LC primary structure characterization workflow using Agilent AdvanceBio RP-mAb columns and Agilent 1260 Infinity Bio-Inert LC system
Now you have more columns and more choices to improve your charged-variant analysis of proteins with the Agilent Ion-exchange BioHPLC columns.
This Application Note shows the workflow solution for the physicochemical and biological characterization of a biosimilar and innovator rituximab.
The charge heterogeneity provides a great challenge for the complete characterization of the monoclonal antibody (mAb).
This work describes a high flow LCMS method to demonstrate the high resolution mass analysis of mAbs with good chromatography and its utility in analysis of Biosimilar mAbs.
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